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Q. After Drosophila DNA has been treated with a restriction enzyme, the fragments are attached to plasmids and selected as clones in E. coli. By using this "shotgun" technique, David Hogness recovered every DNA sequence of Drosophila in a library.
a. How would you identify the clone that contains DNA encoding the protein actin, whose amino acid sequence is known?
b. How would you identify a clone encoding a specific tRNA
These experiments demonstrated that DNA is the genetic material of phage and that protein does not transmit genetic information. Can this same experiment be carried out with 35S and 14C? Describe it.
Earlier in 2010, the Pacific island nation of Samoa decided to switch from driving on the right to driving on the left. Why may these cause difficulties? Why is it complicated for a cell to change codon usage, say, switching a "stop" codon to a trypt..
Test the given hypothesis of the secondary exposure to cigarette smoking during childhood has importance in developing the lung cancer in person.Describe how efficient is surgical treatment for the Ankylosing Spondylitis.
Chromatographic assay reveals the total amount of T3 in the blood is ten micrograms. You discover that the various bound forms of the hormone are.
Maraviroc is a drug designed to block the interaction between HIV attachment proteins and the host cell receptor protein CCR5, thus blocking the initial stages of viral infection.
A reactio mixture containing 20 mM O-acetly serine, fifty mM NaCl, 50 mM HEPES buffer (pH 7.6), and O-acetyl serine is incubated at 37 C until the O-acetyl serine is completely hydrolyzed.
Compare and contrast aerobic respiration, anaerobic respiration, and fermentation and how do antibiotics work? Why do they kill pathogens without harming us?
In cats, the X-linked allele O converts the black pigment found in the fur into an orange pigment. Heterozygous females are mosaics of orange and black pigmented fur.
What hormone do the adrenal glands produce. An uncold-worked brass specimen of average grain size 0.008 mm has yield strength of 170 MPa. Estimate the yield strength of this alloy (in MPa) after it has been heated to 600°C for 1260 s, if it is known ..
A population in which a mutation produces a new allele that increases the individuals that have that allele to have a higher probability of surviving to reproductive age (assume a 5% increase). Assume the mutation entered the population 1 generati..
You have an F- strain of E. coli that is resistant to streptomycin but requires the following amino acids for growth on minimal medium: arginine, cysteine, methionine, phenylalanine, and proline.
In 1958 federal law restricted the movement of soil and plants from areas where fire ants were established. Why was this law ineffective in stopping the spread of fire ant colonies.
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