Reference no: EM131658733
Lab - Introduction to Culturing & Aseptic Technique
Experiment 1 - Agar Plate Preparation and Bacterial Inoculation
Post-Lab Questions
1. Which plate grew the most bacterial species (based on number of different colonies)? Was this a surprise? Why or why not?
2. Was the control plate free of bacterial colonies? If yes, what does this mean for your experiment? If not, what could be the source of the contamination?
3. Did the airborne contamination plate contain any growth? Were any of the colonies similar in appearance to those found on the other plates? How does this plate demonstrate the need for following aseptic technique when culturing bacteria?
Experiment 2- Bacterial Transfer to a Stab Tube and an Agar Plate
Post-Lab Questions
1. Did the colonies that grew on the transfer plates retain the same appearance from the initial plate? Why or why not?
2. Were the colonies uniform in appearance on the transfer? What could account for any differences seen?
3. Did growth occur through the depth of the stab tube? If not, what could account for the lack of bacterial growth?
4. Why was it important to include a control plate and stab tube in this experiment? Did they serve as positive or negative controls?
Attachment:- Assignment File.rar