Which of the following is used to digest proteins in sample

Reference no: EM133224549

Assignment:

PLEASE ANSWER ASAP

1. Why are PCR products pooled and amplified DNA are extracted or purified?

a. To remove contaminants and purify genomic DNA from the amplified DNA preparations

b. To remove PCR components (primers, dNTPs, enzymes, salts, etc.) and other contaminants from the amplified DNA preparations

c. To remove extraction reagents (lysis reagent, wash buffer, etc.) and other contaminants from the amplified DNA preparations

d. All of the choices

2. In genomic DNA extraction in the protocol, which of the following is used to digest proteins in the sample?

a. Proteinase K

b. Elution buffer

c. Carrier RNA

d. Ethanol

3. Which of the following is a correct match?

a. Labelling/staining biofilm: Yeast extract-peptone-dextrose (YPD)

b. Fixing biofilm: 4% formaldehyde or paraformaldehyde in PBS

c. Culturing biofilm: neat methyl salicylate (MS)

d. Clarifying biofilm: Calcofluor White M2R

4. Which of the following is an opportunistic pathogen that causes mucosal yeast infections such as thrush (oral candidiasis) and genitourinary infections?

a. Staphylococcus aureus

b. Candida albicans

c. Lactobacillus acidophilus

d. Escherichia coli

5. The most common Candida species associated with yeast infections is _____.

a. C. krusei

b. C. tropicalis

c. C. aureus

d. C. albicans

6. Which of the following is one of the fungal species shown in the representative result?

a. All of the choices

b. Streptococcus thermophilus

c. Escherichia coli

d. Phanerodontia chrysosporium

7. Which of the following is a correct match?

a. Fixing biofilm: Yeast extract-peptone-dextrose (YPD)

b. Clarifying biofilm: 4% formaldehyde or paraformaldehyde in PBS

c. Culturing biofilm: neat methyl salicylate (MS)

d. Labelling/staining biofilm: Calcofluor White M2R

8. Sequencing of fungal ITS regions allows for the detection of numerous fungal species, specifically some ____________, that often go undetected by traditional microscopy-based method.

a. Basidiomycota

b. Ascomycota

c. Zygomycota

d. None of the choices

Reference no: EM133224549

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