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A type of phosphatase can be assayed using p-nitrophenyl phosphate but the assay is performed at pH 7.8, which is the optimum for this enzyme. The initial velocity measured at the highest substrate concentration was .45 deltaA_400 / minute. Convert this velocity to a change in concentration/minute (microM/minute). HINT Use the pKa of p-nitrophenyl (7.0) and the henderson hasselbalch equation to correct the concentration, since at pH 7.8 not all of the product is in the colored, visible form. Use 18,380 M^-1cm^-1 for the extinction coefficient.
Explain the role of the citric acid cycle as a central metabolic mechanism. Explain what happens to the cells' abilities to oxidize acetyl CoA when intermediates of the cycle are drained off for amino acid biosynthesis.
A patient with hypertension is being treated with hydrochlorothiazide and is due for a twenty-five mg dose shortly. Her latest potassium lab result is 2.4 mEq.
What effect might this have on cell lineages during development. Describe the physical changes that would occur in SIM medium if you inoculated an organism that was negative for hydrogen sulfide but positive for indole production and motility.
Glucose (C6H12O6) can survive as both an open-chain form and a closed-ring form. Before 1900, glucose was only thought to happen as an open chain. Now we know that over 99 percent of the time, glucose occurs in the closed-ring form. What probable dif..
A drug 2,4-dinitrophenol can get into mitochondria, and carry portons from the intermembrane space, and into the matrix. Determine the effect of DNP on the ATP synthesis in the presence of oxygen?
Discuss embryonic stem cell invitro methord in which transgenic animal were generated and efficient show how homologous recombination happening?
As we have discussed in class, individual phylogenetic methods are best suited to particular kinds of troubles. For each of the following data sets, assess the suitability of maximum parsimony to that data set.
Assume you place a cell into a solution containing small/non-polar purple dye molecules and observe it under a microscope.
Determine which chemical groups of nucleotides react to form a covalent phosphodiester bond?
It is not normal to administer a pure drug to a patient. Almost all the drugs are administered in form of a medicine. A medicine is a mixture of one or more drugs combined or formulated with the other inactive materials known as excipients.
A snapdragon with pink petals, black anthers and long stems was allowed to self-fertilize. From the resulting seeds, 650 adult plants were obtained.
Suppose that the fifty resident alligators at Wakulla Springs State Park represent a population that is NOT in Hardy-Weinberg equilibrium
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