Which compound will be accumulated by the double mutant

Reference no: EM13990729

Sexual reproduction occurs in the bread mould (Neurospora crassa) when two haploid strains of different mating types come into contact with each other. The haploid cells fuse to form a diploid cell, which then undergoes meiosis to produce haploid spores. The spores are dispersed and grow into new fungal colonies as haploid individuals.

In Neurospora, two genes participate in the synthesis of valine. The wild-type allele for one gene is designated val-1+ and a mutant allele designated val-1-. The second gene has a wild-type val-2+ allele and a mutant val-2- allele. These two genes are linked, with a map distance between them of 25%.

a. Progeny from the cross: val-1- val-2+ X val-1+ val-2- are plated on minimal medium. What proportion of the progeny will grow on minimal medium?

b. The val-1- val-2+ strains accumulate intermediate compound B, and the val-1+ val-2- strain accumulate intermediate compound A. Draw the two possible linear pathways that will explain these results. Include on your pathway each of the chemicals mentioned in the question and valine. Also indicate which gene product (val1+ product or val2+ product) controls which step.

c. The val-1- val-2+ strains will grow if the minimal medium is supplemented with valine or compound A, however the val-1+ val-2- strain will grow only on medium supplemented with valine and not with compound B. Draw a table to describe these results, indicating the growth abilities of each strain on:

Strain Minimal medium (MM) MM + val MM + A MM + B

d. Indicate which of your pathways in part b is the correct one.

e. Which compound will be accumulated by the double mutant val-1- val-2- ?

Reference no: EM13990729

Questions Cloud

Compute magnitude and angle of inclination with x axis : Determine the resultant of the coplanar concurrent force system shown. Compute the magnitude, sense, and angle of inclination with the X axis. Use the method of components.

How ikea influences all elements of its supply chain : Explain how each sector of industry is linked together within a supply chain. Describe how IKEA influences all elements of its supply chain to adopt sustainable practices

Radon-nikodym derivative : Let µ and ν be probability measures on S such that ν is singular with respect to µ on S, but absolutely continuous with respect to µ on each An with Radon-Nikodym derivative fn . Show that fn → 0 a.s. for µ. Hint: If fn → f a.s., show by Fatou's l..

Nondecreasing sequence of bounded stopping times : Let α be a stopping time and τ (n) a nondecreasing sequence of bounded stopping times, both for a martingale {Xn, Bn }n≥1. Show that α is also a stopping time for the martingale {Xτ (n), Bτ (n)}n≥1, in the sense that {α ≤ τ (n)}∈ Bτ (n) for each n..

Which compound will be accumulated by the double mutant : In Neurospora, two genes participate in the synthesis of valine. The wild-type allele for one gene is designated val-1+ and a mutant allele designated val-1-. The second gene has a wild-type val-2+ allele and a mutant val-2- allele. These two gene..

Determine the maximum pressure behind the dam : Fresh water is impounded behind a dam to a height h of 18 ft, as a shown. determine the maximum pressure behind the dam.

Problem regarding the real-valued function : Show that for any metric space S, if A ⊂ S and x ∈ A\ A, then there is a bounded, continuous real-valued function on A which cannot be extended to a function continuous on A∪{x }. Hint: f (t ) := sin(1/t ) for t > 0 cannot be extended continuously..

Problem regarding the one-point compactification : Let (X, d) be a locally compact separable metric space. Show that its one-point compactification is metrizable.

Contains real numbers of arbitrarily large : If S exists, then S(x, y) = 0 ∈ R. Then by Problem 4 there must be neighborhoods U of x and V of y in K such that S(u, v) ∈ R and |S(u, v)| 1 for all u ∈ U and v ∈ V . Show, however, that each of U and V contains real numbers of arbitrarily l..

User Account

All Pages