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1. Write 1-2 paragraphs describing the work presented. Include in your overview, some background on the topic and the goals and rationale of the study.
2. Write 1-2 paragraphs describing the experimental design used to address the goal/problem and list the major results from each approach. Be sure to include the two key areas (proteomics results and central metabolism results) and how they connected the results from these two areas to make a complete story.
3. Answer the following questions.
1. Given what you know about regulation of central pathways, why did the authors not identify pathways that were required during infection in their proteomics experiments?
2. Could an enzyme/pathway be important during infection but not show an effect when it is knocked-out? Why?
3. What are the main findings in the paper? [hint: what does E. coli eat in the urinary tract?]
4. What is an auxotroph? How did the author demonstrate auxotrophic phenotypes in vitro? What did the in vivo results from the 2 auxotrophs tell us about the urinary tract environment and how did these data support the main findings of the paper?
Attachment:- Journal article for pathways assignment.rar
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
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Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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