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you observe 50,000 RFUs in a 200 microliter aliquot from a G3-500ml culture. How many total RFUs will be present in a 15 mLsample? If you recover 80% of the fluorescing protein during purification, what would the maximum total number of RFUs present in all of your fractions?
The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin..
The resulting f1 generation consists of all tall pink flowered plants. Assume that height and flower colour is each determined by a single gene locus on different chromosomes; predict the results of an f1 cross of dihybrid plants. Select appropriate ..
In humans the presence of chin and cheek dimples is dominant to absence of dimples, and ability to taste the compound PTC is dominant to the inability to taste the compound.
What are the most probable genotypes of the male and female parents? Give details the strategy used to determine the genotypes. What is the probability of the subsequently offspring having a spotted brown tail.
All known human cancers have been demonstrated to be caused by viruses, You have been asked to design a virus that causes cancer in humans. Which of the following cellular functions would this virus have to possess to cause a tumor (select all that..
A miRNA regulates its target mRNA through degradation. Let us suppose miRNA-199 targets Blimp1. Which progenitor population in the developing embryo will be affected?
Assume if your target DNA is 500 base pair long molecule, that is amplified for forty cycles, how many micrograms of DNA product would you get?
ATP synthesis in chloroplasts is very similar to that in mitochondria: Electron transport is coupled to the formation of a proton (H+) gradient across a membrane.
How to use microarray to establish which genes are transcribed in which organs and tissues or different environmental situations?
An insufficiency of antidiuretic hormone can cause an individual to develop diabetes insipidus. What do you forecast the clinical manifestations of this disease to be?
A ct scan of a forty year old woman with parathyroid adenoma and hyperthyroidism reveals extensive calcium deposits in the lungs and kidney paranchyma.
The cell walls if bacteria, fungi and plant cells and an extracellular matrix of animal cells are all external to the plasma membrane. Which is the characteristic of all these extracellular structures.
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