What would shaggys results look like compared

Reference no: EM133442059

Question 1. Shaggy has a dog (Scooby) that has a rare form of skin cancer. He is sequencing Scooby's genome using Sanger sequencing in hopes to help the veterinarian and oncologist design better treatments. He added the DNA template, primers, DNA polymerase, and dNTPs, but not the ddNTPs (dideoxy analogs). What would Shaggy's results look like compared to if he would have performed the sequencing properly with the ddNTPs added?

Question 2. There has been an outbreak of middle ear infections caused by Streptococcus pneumoniae in your neighborhood. The local clinic has been prescribing amoxicillin (an antibiotic) to those infected. Instead of properly disposing of the antibiotics, your neighbors have been dumping amoxicillin in a pond. You hypothesize that amoxicillin is producing antibiotic-resistant bacteria. You obtain the sample from the pond and using selective plating, isolated two strains of Pseudomonas aeruginosa. You want to see if the two environmental strains exposed to the antibiotics produce different proteins compared to the strains found in lung infections. What experimental method would you use and why? [Choose between genomics, transcriptomics, proteomics, or metabolomics]

Question 3- Of the three steps in sequencing a genome (sequence, assembly, annotation), which step is the most labor intensive and why?

Reference no: EM133442059

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