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Assume that a culture of E. coli was grown for approximately 50 generations in 15N (provided in the medium in the ammonium ion), which is a heavy isotope of nitrogen (14N). You extract the DNA from the culture, and it has a density of 1.723 gm/cm3 (water = 1.00 gm/cm3). From the literature, you determine that DNA containing only the common form of nitrogen, 14N, has a density of 1.700 gm/cm3. Bacteria from the 15N culture were washed in buffer and transferred to 14N medium for one generation immediately after which the DNA was extracted and its density determined.
What would be the expected density of the extracted DNA?What band(s) would be expected if subjected to ultracentrifugation through a CsCl gradient?(b) How would your answer to (a) above differ if replication occurred in a conservative fashion?(c) Assume that the extracted DNA was completely denatured (separated into two single strands by heating at 90oC for 15 minutes), what would be the density (or densities) of the DNA (s) in the heated extract?
Would you expect the measurements to be equivalent in both preparations? If a variation in size is expected, how will you account for this difference.
List the three different structures for locomotion found among protozoans, and name an organism taht utilizes each structure.
Such examination would be costly and it would be tricky to ensure that all unforseen consequence was examined. Given issues discuss whether you believe society should continue to invest in the use of transgenic organism, justify your position.
Is habitat temperature reflected in the genomes? What is presumably connected to thermostability.
The boss in your laboratory has just heard of a proposal through another laboratory that genes for eye color & the length of body bristles may be linked in Drosophila .
why the plasma membrane exists in all living things.
Assume you have been examining a new drug that binds to PPAR? and produces its beneficial effect (increased insulin sensitivity). When you give this chemical to mice, you see kidney damage.
What is the importance of membranes in the body? What are the three types of membranes? Compare and contrast each type. What homeostatic imbalances may occur relating to membranes, and what are their implications?
You have an F- strain of E. coli that is resistant to streptomycin but requires the following amino acids for growth on minimal medium: arginine, cysteine, methionine, phenylalanine, and proline.
Which of the following do anaerobic methanogens produce?
The cells that will eventually develop into gamete-producing tissue are typically sequestered away from the developing organism quite early in development.
At 45C, the vapor pressure of water for a glucose solution in which the mole fraction of glucose is 0.080 is 65.76 mmHG. Calculate the activity and activity coefficient of the water in the solution.
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