What will a graph of the effect of ph on the activity

Assignment Help Biology

Reference no: EM13326614

Suppose that an enzyme uses a catalytic mechanism in which a functional group on the enzyme has to accept a proton from the intermediate formed in the first chemical step. The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is 1 X 10 ^5 sec^-1.

1. What will a graph of the effect of pH on the activity of this enzyme look like? (Graph of kcat vs pH)

2. What type of functional group might be responsible for the pH effect on kcat? Justify your anser based on the generic pKa values of functional groups in peptides and proteins..

Reference no: EM13326614

Questions Cloud

What is the separation between the plates : A parallel-plate air capacitor is to store charge of magnitude 290pC on each plate when the potential difference between the plates is 36.0V, If the area of each plate is 6.80 cm2, what is the separation between the plates

Compare and contrast the codes adopted by the ICIFI : Compare and contrast the codes adopted by the ICIFI and by Abbott Laboratories. Assess the codes in terms of utilitarian criteria, in terms of their recognition of moral rights, and in terms of their adherence to standards of justice. What explanatio..

Calculate the area of each plate : The plates of a parallel-plate capacitor are 3.00mm apart, and each carries a charge of magnitude 75.0nC. What is the area of each plate

Obtain the magnitude of the centripetal force : In a skating stunt known as crack-the-whip, a number of skaters hold hands and form a straight line. obtain the magnitude of the centripetal force

What will a graph of the effect of ph on the activity : The pKa of that basic functional group on the enzyme is 8.0, and is the only pKa apparent on a plot of kcat vs pH for the activity of this enzyme. The kcat of the fully active form of the enzyme is 1 X 10 ^5 sec^-1.

Calculate the speed of the stone : A 0.60 kg stone attached to a string is whirled in a horizontal circle of radius 43 cm as in the figure below. The string makes an angle of 35° with the vertical. Find the speed of the stone

What is the frequency of the em wave : In SI units, the electric field in an electromagnetic wave is described by Ey = 100 sin(107x - ?t ). What is the frequency of the EM wave

Understand self-government to mean democracy : "The United States is often described as an experiment in self-government. Most people understand "self-government" to mean democracy. According to this understanding, people are self-governed if they regularly vote to select the individuals who are ..

What is the frequency of these microwaves : A radar unit sends out 0.05 microsecond pulses of microwaves whose wavelength is 25 mm. What is the frequency of these microwaves

User Account

All Pages