Reference no: EM13991308
What was the purpose of washing the red blood cells with saline prior to lysis?
2. What property of the lysis buffer caused the red blood cells to lyse?
3. What are the differences between the primary and secondary antibodies usedin this study?
4. What are the advantages of using indirect immunodetection over directimmunodetection?
5. Antibodies are made when animals detect a foreign antigen. All mammalscarry albumin in their blood. How was a rabbit antibody against albumincreated? Was this lethal to the rabbit? Explain you reasoning.
6. Albumin is not a cytoskeletal protein. What was the purpose of usingantibodies to detect albumin in our cytoskeletal preparations?
7. Why was it necessary to transfer the proteins separated by SDS-PAGE ontonitrocellulose membranes prior to incubation with anti-albumin antibodies?
8. Why did we electrophoretically transfer the proteins resolved by SDS-PAGEonto nitrocellulose? Can you suggest another way to transfer proteins from aSDS gel to a membrane?
9. Why did we wash the nitrocellulose blots with blotting buffer three times afterincubating with the primary and secondary antibodies?
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One cell of the leopard frog
: One cell of the leopard frog (Rana pipiens) has 16.8 billion (16.8 X 109) bp of DNA.(a) If each nucleosome in this cell has a typical amount of "core DNA", and linker DNA that averages 40 bp, how many nucleosomes are there in a leopard frog cell?
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Redeem for additional merchandise
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How are ethical principles objective-subjective or relative
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: (a) If an inductor carrying a 1.50 A current stores an energy of 0.250 mJ, what is its inductance? How much energy does the same inductor store if it carries a 3.1 A current?
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What was the purpose of washing the red blood cells
: What was the purpose of washing the red blood cells with saline prior to lysis? 2. What property of the lysis buffer caused the red blood cells to lyse? 3. What are the differences between the primary and secondary antibodies usedin this study?
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How are phenotypes driven by dna
: How are phenotypes driven by DNA?(inheritance in offspring)
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What is the magnitude of the electric field e at a distance
: An infinitely long solid nonconducting cylinder of radius 2.40m with a uniform charge density p = +1.00 x 10-6 C/m3 has a coaxial infinitely long cylindrical hole of radius 0.80 m. What is the magnitude of the electric field E at a distance r = 0.40 ..
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Alanine, is an amino acid with a relatively small
: Alanine, is an amino acid with a relatively small, non-polar R-group shown here:Would it be able to efficiently cross a lipid-bilayer?
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Find the charge per unit length on the plastic shell
: Concentric with the plastic shell, there is an infinitely long thick conducting cylindrical shell with inner radius a2 = 0.4 m and outer radius a3 = 0.5 m on which there is no net charge. Find the charge per unit length on the plastic shell. Calc..
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