What was the ph at the end of the reaction

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Reference no: EM13184152

An enzyme-catalyzed reaction was carried out in a 0.1 M Tris buffer, pH 7.8. As a result of the reaction, 0.015 mole/Liter of H+ was produced. Note that the pKa of Tris is 8.1.

a) What was the ratio of Tris+ (protonated form)/Tris0 (conjugate base) at the start of the reaction?

b) What were the concentrations of Tris+ and Tris0 at the start of the reaction?

c) What were the concentrations of Tris0 and Tris+ at the end of the reaction?

d) What was the pH at the end of the reaction?

e) What would the final pH be in the absence of any buffer?

Reference no: EM13184152

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