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In a mutant strain of E. coli, a tRNA that recognizes the codon 5' CUG 3' of normal cells has a point mutation so that it now recognizes the codon 5' GUG 3' while still carrying its original amino acid. Surprisingly, a missense mutation found in a protein-coding gene is no longer causing problems for the cell due to the presence of this mutant tRNA.
a. What amino acid is this tRNA carrying?
b. Before the missense mutation in the protein-coding gene, what was the original encoded amino acid?
c. Before the change in the tRNA gene, what missense amino acid was incorporated in protein?
Assume you have 5mL of cells you need to treat with Hydrogen Peroxide, such that the final concentration of Hydrogen Peroxide is 5uM. How much of a 10mM stock solution of Hydrogen Peroxide would you add to your cells?
Propose two possible additional mutations in two separate genes from the receptor that could allow cockroaches with a mutant RTKX to now respond to females. You may consider mutations that could either increase or decrease the activity of the prot..
define a genetic cross between two sufferers
Two NotI restriction enzymes cleave DNA on opposite sides of the Dbm gene in a species of yeast. A molecular probe for Dbm detects a DNA restriction fragment of 8.5 kb in organisms that are wild type at Dbm.
If a pea plant that is heterozygous for round, yellow peas (RrYy) is crossed with a pea plant that is homozygous for round peas but heterozygous for yellow peas (RRYy), how many dissimilar phenotypes are their offspring expected to show.
Determine what is the currently accepted scientific explanation for anthropogenic global climate change?
Discuss five anatomical changes in the vertebrate body plan during the course of vertibrate evolution and describe how each of the changes have influenced the history of Vertebrates.
Concerns surrounding both dehydration and over hydration. Focus on water, sodium and potassium. How reliable is thirst as a guiding factor in fluid intake?
X-linked Recessive: colour-blindness, malepatterned baldness, hemophilia Color-blindness is caused by a recessive gene (b) on X chromosome. Predict phenotypes for children of a female carrier with a normal male. A genetic wasteland.
Some migratory birds are heterothermic, meaning they sometimes maintain constant body temperature and other times permit their body temperature to vary.
what is the theorectical yeild of PH3 when 150 g of Na3P is mixed with 250 grams of water? If the acual yeild is 30 , what is the % yeild?
Cleavage of a peptide by cyanogen bromide and trypsin produces the following fragments. What is the sequence of the peptide?
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