What was the concentration of the original culture

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Questions:

1. You want to make a 10-6 dilution in 100 μL  from a stock of E. coli. How much E. coli stock do you need to add? How much nutrient broth do you need to add as a solvent.

2. If there were 312 colonies of a plate made by spreading 0.1 mL of a 10-6 dilution, what was the concentration of the original culture?

 

 

Reference no: EM133502249

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