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Assume that a given plasmid vector to be used in a cloning experiment contains 4000 base pairs of DNA. Assume also that the restriction endonuclease Cuj cuts this plasmid at the following sites (starting from an arbitrary zero point): 1000, 1500, and 3000. Given complete digestion of the plasmid with the endonuclease so that only linear fragments are produced, what sizes of DNA are expected?
You are to convert the 5 mM fumaric acid in a waste stream into malate using fumarase immobilized on nonporous spherical particles. The enzyme has a turnover number of 23/sec, and a Km of 1.55 mM.
The purpose of this exercise is to demonstrate the comparative effect of UV on three bacterial populations. This could have been accomplished without a cardboard or plastic cover. Why was the cover used.
Elucidate results of all four crosses, using clearly defined gene symbols. describe origin of both of the original prototrophic colonies.
which part of aerobic respiration is oxygen actually used
use chi square analysis to test your model - how could you explain this inheritance at the molecular level.
There is a general agreement that life should have evolved in the oceans originally and only much later invaded land. What factors of the physical environment on the early and evolving Earth support these ideas? Changes in which of these factors were..
A patient has been brought to you who is thought to have a mutation of the DVL1 gene, a human homologue of the Drosophila Disheveled gene.
An inbred mouse that expresses Db and Kb is crossed with an inbred mouse that expresses no class I MHC. The F1 offspring are all DbKbKk.
Explain the process of prokaryotic replication. Pay particular attention to the early stages. In your work, be sure to include the origin of replication, DnaA, components.
Compare and contrast fatty acid synthesis and breakdown with respect to.
Discuss the most important SIMILARITIES & DIFFERENCES between DNA repication and RNA trnascreption?
Alkaline phosphatase is used clinically to help with diagnosis of many diseases. List three conditions, that may be diagnosed by measuring the levels of this enzyme in serum.
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