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You insert a 7 kb segment of mouse DNA into the SV40 chromosome (SV40 DNA = 7.5 kb), creating a recombinant viral chromosome. You then transfect a culture of chimpanzee kidney cells with this DNA. You proceed to isolate intact recombinant viral chromatin from the kidney cells. The chromatin is treated with a mild amount of micrococcal nuclease and analyzed by gel electrophoresis. What size bands would you expect to see?
Such a condition is frequently accompanied by diarrhea, which causes a loss of body water. On the basis of what you have learned about osmotic water flows, describe why diarrhea may occur.
The maltose transporter maltoporin spans the outer membrane of E. coli. It is comprised of 18 successive antiparallel Beta - strands arranged like the staves of a barrel to create a channel through the membrane.
Normal cells need to be attached to the extracellular matrix to survive and proliferate. Explain this in terms of outside-in integrin signaling.
A recipe calls for 10 ml of a 25mM stock of EDTA. However, all you have on hand is a five hundred mM stock. How much more concentrated is what you have relative to what the recipe calls for?
Phenyl-Sepharose resin consiss of a phenyl group attached to a matrix of agarose. Suppose you need to use this chromatographic method to separate a mixture of polypeptides consisting of polyalanine, polyserine, polytryptophan and polylysine.
What ratio of genotypes and phenotypes will you get if you perform the following cross using dogs. the wild type b+ produces black fur, the recessive b allele produces brown fur.
Discuss the criteria for diagnosing puerperal infection. What structures may be involved in a Puerperal infection?
If there are 4000 children born to this generation, how many would be expected to have AB blood under the conditions of Hardy Weinberg equilibrium?
Create the pedigree that demonstrate the familial relationships described in the paragraph above. Use a circle to denote a woman, a square to denote a man and a filled symbol to demonstrate individuals.
What is the habitat of most of the species of Escherichia? How do some strains of Escherichia become pathogenic? What are 2 diseases that are caused by different strains of Escherichia?
The role of O2 in electron transport In mitochondrial electron transport, what is the direct role of O2? a)to provide the driving force for the synthesis of ATP from ADP and Pi b)to function as the final electron acceptor in the electron transport..
What are the effects of a molecule that blocks a sodium-glucose cotransporter?
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