Reference no: EM133194637 , Length: Word count: 2 Pages
Question 1: What are some theoretical and practical motivations for the emergence and development of the inter-disciplinary field of Geomicrobiology in the last 20 years? What are some major findings so far?
Question 2: Comment on why only a small fraction of subsurface microbes are actually culturable (i.e., compare AODC with CFU numbers). What are possible reasons responsible for this? Try to present as many reasons as possible. If most microorganisms from various environments are not culturable, how do we know that they are very diverse?
Question 3: Life-earth co-evolution: what roles did minerals play in the emergence of macromolecules and then life itself?
Question 4: Explain the following: E. Coli cells feeding on glucose grow faster when the culture is highly aerated than when the culture is supplied with NO3- (NO2- is produced). What will happen if you give fumarate instead?
Question 5: The following is a series of coupled electron donors and electron acceptors. Using just the data given in the electron tower, order this series from most energy-yielding to least energy-yielding. H2/Fe3+, H2S/O2, methano/NO3- (producing NO2-), H2/O2, Fe2+/O2, NO2-/Fe3+, and H2S/NO3-.
Question 6: Can the plate method be used to isolate a single species from a mixture of two or more? If so, how do you do that? How do you confirm what you get is indeed a pure isolate that you can deposit to a culture bank such as ATCC?
Question 7: Give one specific example to show the mutual interaction (co-evolution) between the geosphere and biosphere.