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Your first project in your new lab is to purify the protein Rock Band (RB) which is known to be phosphorylated by the kinase Heavy Metal Drummer (HMD). As the new kid on the block, you are tasked with purifying tons of HMD for the lab to use. You first attempt to purify the kinase using ion-exchange chromatography. You scour PubMed and determine that the isoelectric point (pI) of HMD is pH 5.5.
(i) What should the pH of your buffer be if you wanted to bind HMD to an anionexchange column? Why?
(ii) What should the pH of your buffer be if you want to bind HMD to a cation-exchange column? Why?
(iii) What potential issues might arise if the pH of your buffer is too close to the pI of your protein of interest? Why might an extreme buffer pH (< 6 or > 9) be unsuitable?
The smallest atoms can themselves exhibit quantum mechanical behavior. Calculate the de Broglie wavelength (in pm) of a hydrogen atom traveling 475 m/s
A flask that can withstand an internal pressure of 2491 torr, but no more, is filled with a gas at 21.0°C and 758 torr and heated. At what temperature will it burst?
Calculate the number of grams of KClO3 needed to make 400 mL of 0.913 M solution.
sulfur dioxide is an unwelcome result of burning soft coal in power plants. Some of the sulfur dioxide ends up as sulfuric acid ( H2SO4) in acid rain.
Bismuth oxide reacts with carbon to form bismuth metal: Bi2O3(s) + 3C(s) --> 2Bi(s) + 3CO(g)
In the acid-base reaction between Sr(OH)2 and HNO3, 0.75 L of 0.85 M Sr(NO3)2 is formed upon the reaction of 0.515 L of HNO3 solution and excess Sr(OH)2.
Show that there are 1.0 X 10^22 (10 to the 22nd power) carbon atoms in a 1-carat pure diamond that has a mass of 0.20 g.
Consider 25.0 g of water at 21.7°C that are mixed with 25.0 g of water at 43.3°C in a foam cup calorimeter. The equilibrium temperature reached was 30.4°C.
Ethane C2H6 burns in oxygen a.) what are the products of reaction b.)write the balanced equation for the reaction
what are the names of the quantity measured by each of seven SI base units and the SI symbols of the unit?
If 10.7mL of .105 M NaOH is required to reach the first equivalence point of a solution of citric acid, how many mL of NaOH are required to completely neutralize this solution
The solubility of oxygen in water is 5.00M when the partial pressure of oxygen is 1.00 atm. Calculate the solubility of oxygen in water in contact with air
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