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Question: One of the original mutations isolated by Jacob and Monod was O0 (also called O-), often caused by single base pair changes. The O0 mutant fails to express B-gal and permease under any condition. Although we now connect the symbol O, operator designation to the binding site of the lac repressor, originally the O locus encompassed the whole lac promoter region. It was shown later that the O0 mutant can bind both CAP and the lacI product. What positive regulator was affected by the O0 mutation and what could a single bp change affect in this mutant?
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