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Q1. What are the principles and the purpose of using differential salt precipitation in protein purification?
Q2. 6-phosphogluconate dehydrogenase has a pI of 6. Explain why the buffer used for a chromatography on DEAE-cellulose must have a pH greater than 6 but less than 9 in order to ensure the enzyme is efficiently bound to the column?
Q3. We load a DEAE-cellulose column adjusted to a pH of 6.5 with the following mixture of proteins: ovalbumin (pI = 4.6), urease (pI = 5.0), and myoglobin (pI = 7.0). The proteins are eluted first with a buffer of weak ionic strength at pH of 6.5, and then the same buffer containing increasing amounts of sodium chloride is used to elute the proteins. What order are the proteins eluted?
The ethylenediaminetetraacetate ion, H2Y 2-, forms a strong complex with the calcium ion. How does the addition of the H2Y 2- affect the molar solubility of Ca(OH)2? Explain.
Explain a solution of 2.00g of para-dichlorobenzene (a clothes moth repellant) in 50.0g of cyclohexane freezes at 1.05°C. Explain what is the molar mass of this substance
a 24.6 ml volume of hcl solution reacts completely with a solid sample of magnesium carbonate producing 183 ml of
Solve for the new pressure in each of the following, with n and V constant: 1)A gas with a pressure of 1.25 atm at 90 Celsiusis cooled to -17 Celsius. 2)A sample of N2 with a pressure of 790 mm Hg at -75 Celsius is heated to 34 Celsius
the combustion of a 0.519 g sample of the compound yields 1.24 g of CO2 and 0.255 g of H2O. What is the empirical formula of the compound?
Calculate the change in entropy that occurs when 18.66 g of ice at -14.6°C is placed in 54.05 g of water at 100.0°C in a perfectly insulated vessel.
Identify the hypothesis of the statement. If both parents have red hair, then their children have red hair. Both parents have red hair.
Write structures for the carbonyl electrophile and enolate nucleophile that react to give the aldol below. You do not have to consider stereochemistry. Draw the enolate nucleophile in its carbanion form.
A particular sample of red brass consisting of 81.0% Cu and 19.0% Zn by mass has a density of 8780 kg/m^3. What is the molality of Zn in the solid solution?
what is the strongest type of intermolecular force that exists between 2 CH3 molecules--hydrogen bonding -Temp dipole or -dipole-dipole
The only materials on hand are 300 mL of white vinegar (5.00% by mass acetic acid, density = 1.006g/mL) and potassium acetate. How should the student proceed?
if 60.2 grams of hg combines completely with 24.0 grams br to form a compound what is the percent composition of hg in
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