What of the would be a the best choice of a cloning vector

Assignment Help Biology
Reference no: EM13110329

You want to study the effect of a mutation in your favorite gene in a cell. You do not plan to purify the gene product, just look at the mutation in vivo. What of the following would be a the best choice of a cloning vector?

a. a vector that overexpresses YFG
b. a vector that allows you to fuse YFG with a 6xHIS tag
c. a single copy vector ( I think this is the right answer)
d. a multicopy vector

Reference no: EM13110329

Questions Cloud

Example of hypothesis test : Provide an example of a hypothesis test you could perform at work or at home. State what the Null and the Alternative hypotheses would be in your test.
When we graph the cfus/ml : When we graph the CFUs/ml and absorbance on the same graph, we would hope to see an upward trend for both. Sometimes the absorbance continues to rise after the CFUs/ml level off into the maximum stationary phase. What would be the cause of that?
Suggest strategies for evaluating hrm technologies : Suggest strategies for evaluating HRM technologies financial impact of talent management initiatives.
Determining student query-statistics : A wholesaler has recently developed a computerized sales invoicing system. Prior to implementing this system, a manual system was used. The distribution of the number of errors per invoice for the manual system is as follows
What of the would be a the best choice of a cloning vector : You want to study the effect of a mutation in your favorite gene in a cell. You do not plan to purify the gene product, just look at the mutation in vivo. What of the following would be a the best choice of a cloning vector?
Setting up study on depression treatments : Describe your selected research issue, problem, or opportunity. Formulate both a numerical and verbal hypothesis statement concerning your selected research issue, problem, or opportunity.
How many liters of hydrogen gas were produced : A volume of aqueous hydrochloric acid containing 0.500 mol of HCl and 0.100 mol of magnesium metal were combined and reacted to completion. How many liters of hydrogen gas were produced under STP conditions?
Experimental car bumper and repair incidents : An experimental bumper was designed to reduce damage in low-speed collisions. This bumper was installed on an experimental group of vans in a large fleet, but not on a control group.
Describe why ordinary least squares solution is not unique : Describe why ordinary least squares solution is not unique. What can you say about residuals of any solution? Is ridge regression solution unique? Explain why or why not?

Reviews

Write a Review

Biology Questions & Answers

  Discussion on microbial death

Describe precisely what is microbial death? Why does a population of microbes not die instaneously when exposed to an antimicrobial agent

  Multiple choice questions - life science

During the Calvin-Benson cycle, in the red Columbine plant, high-energy electrons are transferred into a newly made three carbon compound.

  Why people addicted to methamphetamines

Explain why people addicted to methamphetamines often sleep very little, suffer extreme mood swings, and become very thin.

  Determine the chance for male offspring

You have two flies that have the following traits. The female fly is dominant homozygous for red eyes and she has a rate immune deficiency for which she is recessive homozygous, having one allele on each X-chromosome.

  Identify these as one very sharp prick

While the nurse applies the sharp point of the pin on his arm several times, he is only able to identify these as one "very sharp prick." What would be the most correct explanation for this.

  How several different tripeptides

If there are 20 amino acids commonly found in protiens, how many different dipeptides are there.

  Explain what effect mutating these two codons

In the presence of high intracellular concentrations of tryptophan, only short transcripts of the trp operon are synthesized because of attenuation of transcription 5' to the structural genes.

  Discussing the skeletal system

Which of the following statements is true? The first cervical vertebra is called the axis.The spinal cord protects the vertebral column.

  Inhibition of the disenfectants or antiseptics

I just did a lab with antiseptics and disinfectants and a lab report question asks "were any microorganims increasing within the zone of inhibition of any of the disenfectants or antiseptics?

  Mitochondria of mutant yeast strain

The mitochondria a of mutant yeast strain is discovered to oxidize NADH even in the absensce of ADP. How would the P/O ratio of this mutant compare to the wilfe type?

  Recombination frequency between brillig and slithy genes

The brillig, slithy and vorpal genes all are located on chromosome VII in F. bandersnatchi. Their positions relative to each other are shown below.

  Create the mrna sequence

A section of the E. coli genome that encodes the pheM protein. Write out and label: the reverse complement, the mRNA sequence.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd