What number of bases is deleted in the coding region

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Reference no: EM13112490

Two E. coli genes, A and B, are known from mapping experiments to be very close to each other. A deletion mutation is isolated that eliminates the activity of both A and B. Neither the A nor B protein can be found in the mutant, but a novel protein is isolated in which the amino-terminal 30 amino acids are identical to those of the B gene product and the carboxy-terminal 30 amino acids are identical to those of the A gene product.

a) With regard to the 5' - 3' orientation of the nontranscribed DNA strand, is the order of the genes AB or BA?

b) What number of bases is deleted in the coding region? How do you know?

Reference no: EM13112490

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