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Two E. coli genes, A and B, are known from mapping experiments to be very close to each other. A deletion mutation is isolated that eliminates the activity of both A and B. Neither the A nor B protein can be found in the mutant, but a novel protein is isolated in which the amino-terminal 30 amino acids are identical to those of the B gene product and the carboxy-terminal 30 amino acids are identical to those of the A gene product.
a) With regard to the 5' - 3' orientation of the nontranscribed DNA strand, is the order of the genes AB or BA?
b) What number of bases is deleted in the coding region? How do you know?
DNA represents the architectural blueprint for all living systems, encoding specific instructions in sequence of its four nitrogen having base pairs that are necessary for building organism.
Suppose that the cells grow at about 10^5 cells per square cm and contain about 10pg of RNA per cell, what is the theoretical yield of RNA?
Assume you learned in class that DeltaG for compete oxidation of glucose is -686kcal/mole. According to the DeltaG for ATP and the numbers of ATP,
The unfenced control plot had not changed significantly in species composition. By means of the concepts discussed in the chapter, what do you think happened.
Discuss the differences between bird and mammalian reproduction, both in anatomy and physiology and Define and compare and contrast populations, communities, and ecosystems.
Discuss how a myocardial infarction could occur and explain how drugs, angioplasty and coronary bypass can help the patient.
The symbol + indicates that wild-type recombination occurs, and - indicates that wild types are not formed. Use the data to construct genetic map of the order of point mutations, and indicate the segment deleted by each deletion mutation.
Proteins discusses the different orders of protein structure. Explain why do you think it is that you can cook a pot roast where the meat proteins are altered by heating.
You are investigating YFP, a transcription factor that binds directly to DNA. You know that it recognizes the sequences.
In plant communities today, leaf morphology is correlated with mean annual temperature, so paleobotanists use fossil leaf morphology to estimate the mean annual temperature of paleoclimates.
Dogs (Canis lupus familiaris) and gray wolves (Canis lupus) can interbreed to produce viable, fertile offspring. These species shared a common ancestor recently.
Calculate the amount of ATP equivalents produced by total catabolism of adenosine compare this with ATP equivalents produced by total catabolism glycine.
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