What might prevent a restriction enzyme from cutting

Assignment Help Chemistry
Reference no: EM13687202

Question- What might prevent a restriction enzyme from cutting the DNA of the organism from which they are derived?
Example the EcoRI restriction site is present in a plasmid but experimental evidence shows no cutting of the DNA after the usual protocol is followed?

Provide complete solution

Reference no: EM13687202

Questions Cloud

Describe the similarities and differences between dna : Question- Describe the similarities and differences between DNA gel electrophoresis and SDS-PAGE. Provide at least 3 differences.
Calculate the change in internal energy system : Question- Calculate the change in internal energy of the following system: a balloon is cooled by removing 0.657kJ of heat. It shrinks on cooling, and the atmosphere does 382J of work on the balloon.
What kind of chemical reaction does a restriction : Question- What kind of chemical reaction does a restriction enzyme carry out. Which bond in the DNA does this class of enzymes target. Define the answer of the above problem
Calculate the ph of a 0.00750 m lithium cyanate solution : Question- Calculate the pH of a 0.00750 M lithium cyanate solution, LiCN solution. Ka for Cyanic acid, HCN , is 3.5 × 10-4.
What might prevent a restriction enzyme from cutting : Question- What might prevent a restriction enzyme from cutting the DNA of the organism from which they are derived
Calculate kc and kp for the reaction 2brcl : Problem- Calculate Kc and Kp for the reaction 2BrCl -gives Br2+ Cl2.All in gas phases. If at 500K, the equilibrium concentrations of BrCl, Br2, and Cl2 are .131, 3.51 and .156M respectively
Problem draw the structure of the product : Problem Draw the structure of the product that is formed when the Alkenes- Oxidative Cleavage of cyclohexene is treated with the following reagents-
Calculate n2 solubility in blood as a function of water : Problem- Assume that blood is mostly water. The Henry's law constant for N2 in water at 300 K is 86,000 atm. The hydrostatic pressure acting on a diver is 1 atm at the surface, and increases by approximately 1 atm for every 33 ft of depth.
Complex ion formation constants (kf) for guidance : Problem- Construct a possible flow diagram below for the separation scheme for a solution containing Ag+, Ni^2+, and Zn2+. You may use HCL, NaOH, and Na2CO3 as reagents. Use the solubility rules and the tables of solubility product constants (Ksp)..

Reviews

Write a Review

Chemistry Questions & Answers

  Steps in the mechanism for the following reaction

Show all the steps in the mechanism for the following reaction, When benzene is mixed with deuterated sulfuric acid, deuterium is slowly incorporated onto the ring. Show the mechanism for this reaction and explain how this relates the sulfonation of ..

  Prior to placing piece of metal into the graduated cylinder

This assignment inhibits chemistry Laboratory Questions.

  Write the structures of the saytzeff elimination

Write the structures of the saytzeff elimination

  Calculate ph - chemistry questions

Chemistry Questions on Calculate P H

  How many mols of hydrogen can produce

how many mols of H 2 can produce

  Analysis of corrosion mechanisms

Analysis of corrosion mechanisms and preventative measures

  Chemical and pharmaceutical science

Write an equation for the formation of an acetal from reaction of excess methanol with benzaldehyde in the presence of an acid catalyst.

  Calculate the approximate sulphur

Calculate the approximate SO 2 mass emission in lb/day.

  What is the structure - stereochemistry

What is the structure (including functional groups)? Stereochemistry (racemic or single enantiomer)?

  Design a qualitative analysis scheme

Design a qualitative analysis scheme

  What will be the resultant pressure

What will be the resultant pressure when the stopcock is opened?

  The 1h nmr spectrum

Integrals for some of the resonances in the 1H NMR spectrum are higher than they should be due to the shear number of hydrogens in this compound

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd