What is total yield of dna

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You have isolated a plasmid from a culture of E. coli using the Qiagen Midi kit. You prepared a 40X dilution of the isolated DNA and measured the A260 in the spectrophotometer of this dilution and observed a value of 0.21.

If the extinction coefficient of DNA is 1 ABS unit = 50ng/ul, what is the concentration of the original solution of DNA? (i.e. the solution you have at the end of the midi prep protocol).

If you have 200ul of the solution described above, what is your total yield of DNA? (the amount of DNA in the tube)

Reference no: EM132797203

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