Reference no: EM13927421
Fluorescence is emitted from a single fluorophore with a lifetime of τ. The idealized exponential decay is indicated by the dottedline in Figure
1. However, you have a detector with a signifcant exposure time ?t, such that the fluorescence level changes during the exposure. Your setup allows you to trigger two such exposures D1 and D2, indicated in the figure. As an experimenter, you know the two measurements began (were triggered) at 0.8 and 2.5 nsec, but you do not know ?t, the exposure time (the areas in the figure are just for illustration).
You have a linear array of 51 such detectors. On the website, you can download a file called FLIMdata.dat, a 2-by-51 matrix that represents D1 and D2 measurements at each detector. Plots of the D1 and D2 intensities versus detector position (1 through 51) are shown in Figure 2. Your job is to make a plot of τ , the fluorescence lifetime, versus detector position (which would correspond to, for example, a linear cut across a region of cells).
This will require some open-ended thinking about how to extract τ from the measured data. You should find that the calculated lifetime is much more spatially uniform than the noisy intensity data, with a single well-defined region of spatial contrast. What is the uniform background lifetime, in nanoseconds, and what is the lifetime in the contrasting region?
Attachment:- Flim.m.txt
|
Inventory systems summary
: The principal role of an inventory management system is to report and track inventory in various companies, such as in retail. There are different types of inventory systems that can be used by a company such as First-In-First-Out (FIFO), Last-In-..
|
|
What is the statistic used developing the decision
: 01. What is the statistic used developing the decision rule for each of the following problems. If the statistic used is the student t distribution you must indicate the sign or signs of the critical t value.
|
|
What are four basic purpose of financial statements?
: What is the primary purpose of each of the four basic financial statements?
|
|
Performance of personal and business investments
: The performance of personal and business investments is measured as a percentage, return on investment. What type of variable is return on investment?
|
|
What is the uniform background lifetime
: You should find that the calculated lifetime is much more spatially uniform than the noisy intensity data, with a single well-defined region of spatial contrast. What is the uniform background lifetime, in nanoseconds, and what is the lifetime in ..
|
|
Compare and contrast terrorism and terrorist groups
: Compare and contrast terrorism, terrorist groups, organized crime, and their financing activities and practices. Analyze the role of intelligence and counterterrorism activities among federal agencies.
|
|
What is the asymmetric information concept
: What is the asymmetric information concept? What role does this concept play in a company's decision to change its financial structure or issue new securities?
|
|
Best practices for customer relationship management
: Business leaders who understand how supply chain management directly affects customer satisfaction look at key processes within the supply chain for improvement because these processes are key to improving the organization's ability to meet custom..
|
|
Goodness of fit test and contingency tables
: Births Records of random selected births were obtained a categorized according to the day of the week that they occurred (based on data from the National Center for Health Statistics). Because babies are unfamiliar our schedule of weekdays, a reas..
|