What is the total volume of phage

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An SDSU junior has grown a bacteriophage stock during a BIOL 350, General Microbiology, lab. He determines it has a concentration of 7.5 x 1014 phage/mL. He is given a Salmonella typhimurium host culture with a concentration of 8.1 x 1012 cells/mL and adds a 300 uL aliquot of his bacteriophage stock to 5 mL of Salmonella cells.

a. What is the m.o.i. in phage/cell? Answer to one decimal place.

b. What is the total volume of his phage + host cell mixture? Answer to one decimal place.

c. What volume, in microliters, of bacteriophage stock should he add to 3 mL of host cells to have a 0.7 moi? Answer to the nearest whole number.

Reference no: EM131967186

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