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Question 1: Design a 12 bp forward and a 15 bp reverse primer manually (don't use online primer design tool) which can amplify the DNA fragment whose sequence is highlighted above. Write the primer sequences below. No need to check any other criteria for primer efficacy.
Question 2: What is the size of the amplicon after 30 rounds of PCR amplification using the above primers?
Question 3: How much minimum extension time in seconds is recommended to generate the amplicon using the above primers? Assume that the thermostable DNA polymerase copies at a rate of 1kb/min.
You want to generate a mutated version of the above amplicon. Using site-directed mutagenesis, you want to change the nucleotide at the 7831st position from G to an A. Write the sequence of your PCR forward and reverse primers which can be used to generate the desired fragment. Do not use an online primer design tool (may choose any length while designing the primers).
Question 5: Do you think the extension time needs to change to do the site-directed mutagenesis mentioned in part d above? Explain your reasonings.
Question 6: How to check if the base change happened after the PCR cycles?
Please provide a complete mechanism (using arrows and lone pairs) for the synthesis of 2-ethyl-2,4,5-trimethyl-1,3-dioxolane using 2-butanone and 2,3-butanediol in an acidic environment (H2SO4).
Draw an ideal distillation curve (temperature vs. milliliters of distillate) for a 50:50 mixture of two miscible liquids. How might one achieve such an ideal distillation
Attached: When 102 J of energy are added to a sample of gallium that is initially at 25.0 oC, the temperature rises to 42.0 oC. What is the volume of the sample in cubic centimeter?
15.00 g of solid ammonium hydrogen sulfide is introduced into a 500.-mL flask at 25°C, the flask is sealed, and the system is allowed to reach equilibrium. What is the partial pressure of ammonia in this flask if Kp = 0.108 at 25°C for
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when 5mls of 0.05m ch3cooh is allowed to react with 1.5mls of 0.05m koh. the ph is found to be 4.43.using the
How many moles of ions are in 1.60 mol of K2SO4?
What volume of gas should you collect at 25 degree celsius and 1.00 atm in order to achieve the desired pressure in the cylinder?
How do I calculate the viscosity average and number average molecular weights of polyvinyl alcohol given the molar mass of cleaved and uncleaved polymer solution. (Also given intrinsic viscosity concentration, other experimental data)
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At a certain temperature the vapor pressure of pure benzene and pure toluene are 380 torr and 130 torr, respectively. If 7.50 mol benzene is mixed with 6.64 mol toluene
a cylinder of compressed gas is labeied composition mole 4.5 h2s 3.0 co2 20 co remaainder n2. the pressure gauge
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