What is the purpose of the 30 minute incubation

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Reference no: EM133435765

Questions:

1. You are working to transform E. coli bacteria with a plasmid that contains a gene whose expression makes bacteria antibiotic resistant. On plate A, you spread out E. coli that was transformed with the plasmid on agar containing the antibiotic. On plate B you spread out E. coli that was transformed with the plasmid on agar that does not contain the antibiotic. With respect to bacterial growth, what do you expect to see on each plate and explain why?

2. What is the purpose of the 30 minute incubation step in the recovery broth? What is missing and why is it missing from the recovery broth that is present in the agar plates used to select for transformed bacteria?

3. Provide an example of where in "nature " could transformation be occurring and possibly lead to transfer of genetic information that makes a pathogen more harmful? Be as specific as possible. Think about an environment where lots of bacteria can be interacting. Provide an example of the genetic information that might be transferred in this environment that could lead to a more harmful pathogen?

Reference no: EM133435765

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