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What is the purpose of smear preparation

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Questions

1. What is the purpose of smear preparation?

2. What is a simple stain useful for observing?

3. What are the three most common bacterial shapes?

4. Simple staining is used to visualize what part of the bacterial cell?

5. What will happen if too many bacteria are added to the smear?

6. What charge does a negative stain dye have?

7. What is the charge of a bacteria cell?

8. Why are negative stain smears not heat fixed?

9. What is a glycocalyx?

10. What two areas are stained during a capsule staining?

11. What is a differential stain?

12. List and describe the four major steps of gram staining.

13. What is a mordant and which agent acts as the mordant in the gram-stain?

14. Which agent acts as the counterstain in the gram-stain?

15. What is the difference between a gram - and a gram + bacteria?

16. What is an endospore?

17. What is the endospore stain classified as?

18. What is used as the primary stain in this procedure?

19. What is used as the counterstain in this procedure?

Reference no: EM133313678

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