What is the purpose of pour plates technique

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Reference no: EM133231596

Assignment:

1. What is the purpose of pour plates technique?

2. Would you choose to employ the pour plates technique for an original sample with small or large number of viable bacteria? Explain.

3. Consider adding 1 g of breakfast burrito into 999 ml of water. Calculate 1,000-fold dilution for five consecutive tubes. Explain the dilution created for each tube (A, B, C, D, and E) by using a dilution scheme.

4. Why were you instructed to select the plate that contains 30-300 CFUs to count? What does TNTC stands for? What about TFTC? What is the formula to calculate the concentration of bacteria in the original sample (CFU/g)?

5. Suppose that in the plate "D" from exercise #3 above you counted 293 colonies. What is the concentration of bacteria in the original sample? Show your rationale and make sure that the result is expressed in "two-significant figures". Do not forget of variables units as showing them in your rationale, such as "colonies" and "CFU/ml" or "CFU/g".

Reference no: EM133231596

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