What is the purpose of each of these three markers

Assignment Help Biology
Reference no: EM1398504

In a yeast plasmid shuffle, you use three different selectable markers in your strain at the time you are screening for the conditional alleles of interest. The first marker is in the chromosome, the second marker is on the plasmid that carries the wild type copy of the gene of interest, the third marker is on the plasmid that could potentially carry the mutant allele of interest.

A. What is the purpose of each of these three markers? What is the purpose of the plasmid that contains the second marker?

B. One of these three markers has to have a special property that is not needed in the other two markers. What is this property, which of the three markers possesses it, and why is this important?

Reference no: EM1398504

Questions Cloud

What relationship would you expect in the biomass : Eastern hognose snakes are dietary specialists feeding almost exclusively on toads of the genus Anaxyrus. If you conducted a study of these species at Rock Bridge State Park would you expect the number of snakes in your study area to be large, sma..
Explain main ways to show illustration of self-improvement : What motivates him toward this attempt? Does he strike you as self-promoter or genuinely benevolent man desirous of helping others?
Mean number of infected children per sample : If samples of size 224 were repeatedly selected from the population of children born to mothers with HIV antibody, what would be the mean number of infected children per sample?
Aim and obejectives of factor analysis : Critically illustrate out the aim and obejectives of factor analysis? Critically illustrate out the aim and obejectives of Cluster analysis? Critically illustrate out the aim and obejectives of Multidimensional analysis?
What is the purpose of each of these three markers : One of these three markers has to have a special property that is not needed in the other two markers. What is this property, which of the three markers possesses it, and why is this important?
Define homozygous deletion : What is a homozygous deletion? I am doing a research paper on claudin 4 tight junctions. Just want to get a better and more detailed idea of what exactly is a homozygous deletion.
Journal of management information systems : In an article in the Journal of Management Information Systems, Mahmood and Mann investigate how information technology (IT) investment relates to company performance.
What are the brief and basic steps : What are the brief and basic steps that are widely believed to have resulted in the origin of the 1st bacteria from nonliving precursors?
Factor analysis-cluster analysis-multidimensional analysis : Provide an example of a situation in which factor analysis, cluster analysis, and multidimensional analysis might be used.

Reviews

Write a Review

Biology Questions & Answers

  Create a vector or plasmid in class

Jimmy, a new undergraduate researcher in the lab, is told to make a vector having a copy of the CELLBIO gene from Dr. Acosta's genome as well as a selectable gene, Ampr.

  Determine rationale for the medications

An eighty nine year old woman has a long history of systolic heart failure that resulted in a large left ventricular infarct when she was in her 70s.

  Provide physiological reason for dissatisfaction

You are trying to find a restaurant to take your great grandmother for Mother's Day. You have taken her to several places in town, but she is rarely satisfied with the food she is served, complaining that the food lacks flavor.

  How various real map units are indicated

If you obtain an RF value of 45 percent in one experiment, what can you say about linkage? (The actual figures are 58, 52, 47, and 43 out of 200 progeny)

  Find surface area and volume

Determine the Surface Area (cm sq) and volume (cm cu) of the following three cubes.

  Destroying microbes

Explain specifically how irradiation using the following methods destroys microbes provides an examples(s) of materials that can be sterilized by each of the techniques below.

  How will the procedure result in purification of tubulin

GTP is again added to the redissolved pellet proteins, and the solution is again warmed for a few minutes. Then centrifugation at similar speed described above is again performed. This process is repeated several times.

  How enzymes provide energy for the reactions they catalyze

how Enzymes provide energy for the reactions they catalyze

  Is the population in hardy-weinberg equilibrium

MN blood types were assessed for a population that consists of 100 individuals: 45 have blood type M, 30 have blood type MN and 25 have blood type N. Is the population in Hardy-Weinberg Equilibrium with regards to this locus? Show your calculation..

  Base substitution of nucleotides

Discuss how insertion, deletion, and base substitution of nucleotides can have a significant impact on genes. Find three specific examples of ways in which a very small change in DNA

  Describe an application for over expression of siderophores

How can altering ice nucleation protein production yield a bacterial strain useful to agriculture? Explain the logic and how this works.

  Discuss characteristics of the cervical region

What are the characteristics of the cervical region of the vertebral column in amphibians, reptiles, birds and mammals?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd