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Explain the reason for each of these steps in the extraction of DNA.a. What is the purpose of adding detergent to the solution?b. What is the purpose of adding isopropanol to the solution?c. Why do you suppose cold isopropanol is used rather than isopropanol at room temperature?d. What is the purpose of filtering the solution? What is being filtered out?
Based on the Nernst equation, the equilibrium potential for any ion distributed across a membrane is dependent on the internal and external concentrations of the ion,
Suppose when looking at a slide of 3-crossed threads, red is on bottom, and yellow on top. When the adjustment knob is rotated forward on your microscope.
Who was Carl Weigert? What influence did Weigert have on Ehrlich. Other than the acid-fast stain, what other technique may be used to diagnose tuberculosis? What scientist developed this test.
Gene R and gene T are genetically linked. Answer the following questions concerning a dihybrid organism with the genotype Rt/rT.
Where in nature would the levels of oxygen and carbon dioxide be most similar to the candle jar?
Is a viable count really reflective of the number of bacteria in environment you sampled? For example, if grow bacteria in different dilution factors on a agar plate.
Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase.
What function of intermediate filaments have in eukaryotic cells.define structure of microfilaments and microfilament network.
How do you explain why enhancers can be moved around within the DNA without affecting their activity, whereas the TATA box can only operate at one specific site?
2-experimental varieties of strawberry are produced by crossing a hexaploid line that contains 48 chromosomes and a tetraploid line that contains thirty-two chromosomes.
a 45-year old lawyer presents to you office complaining of sporadic cough, fatigue, and low-grade fever for the past seven days. ten days prior, he attended a convention in chicago where the air conditionaer was set at 68 f.
How does an organism with a capsule such as K. pneumoniae, provide the organism with pathogenic abilities to avoid destruction by the host cell?
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