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What is the purpose of a dna ladder

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Reference no: EM132706541

How many base pairs are found in a piece of DNA that is composed of 36 nucleotides?

  1. What is the purpose of a DNA ladder? 
  2. Why is it harder to conduct plasmids through a gel than linear DNA? List the three conformations that plasmids may occur in.
  3. Explain how positive and negative charges are important when performing gel electrophoresis.
  4. What is an allele ladder? What is its function in DNA profiling?
  5. What is required to visualize DNA following electrophoresis?
  6. What is the purpose of the running buffer? 
  7. You are running a gel with DNA fragments that are 150bp, 500bp, and 1200bp in size. Which fragment will travel the furthest? Which will travel the least? Why?
  8. Name two macromolecules other than DNA that can be separated for analysis by gel electrophoresis

Reference no: EM132706541

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