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A pesticide inhibits the activity of a particular enzyme, which can therefore be used to assay for the presence of the pesticide in an unknown sample.
a) In the laboratory, the initial rate data shown in the table below were obtained. What type of inhibitor (competitive, uncompetitive, non-competitive) is the pesticide? Determine the values of Vmax, KM, and KI. v, M/min x 106 S, M No inhibitor 10-5 M inhibitor 3.3 x 10-4 56 37 5.0 x 10-4 71 47 6.7 x 10-4 88 61 1.65 x 10-3 129 103 2.21 x 10-3 149 125
b) After 50 ml of the same enzyme solution in part (a) is mixed with 50 ml of 8x10-4 M substrate and 25 ml of a test sample, the initial rate observed is 18 µM/min. What is the pesticide concentration in the test sample assuming no other inhibitors or substrates are present?
A 1-liter container of nitrogen gas (N2) at a temperature of 23c is intially at a pressure of 1.25 atm. because of a slow leak the pressure falls to 1.15 atm 1 hour later what is the leak rate in molecules/second?
A vapor rate of 2500 kg/h is required and the vaporizer will operate at 6 bara. Size the vessel to restrict the carryover of liquid droplets. The liquid hold-up time need not be considered, as the liquid level will be a function of the thermal des..
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If this machine is operated from a 440-V dc supply, determine the speed at which this motor will run and the current taken from the supply, assuming no mechanical load, no friction and wind age losses, and that the field current is maintained at 4..
Chemical X costs $0.09 per pound. Chemical Y costs $0.04 per pound. Chemical Z sells for $0.80 per pound. If one-half of the selling price for chemical Z is due to costs other than for raw materials, what is the maximum profit obtainable per pound..
Her model indicated that if Stogy hired two rollers per week during the entire first year, inventory would stand at 2,697,000 cigars by year's end.
1. It is desired to use 28.32 m 3 /min of air (metered at a pressure of 101.3 kPa and 294.1 K) in a process. This amount of air which is at rest enters the fan suction at a pressure of 741.7 mm Hg and a temperature of 366.3 K and is discharged at a p..
Styrene can be made by catalytic dehydrogenation of ethyl benzene. The reaction is usually carried out in the presence of steam, which acts as a heat carrier and reduces coking on the catalyst.
An evaporator is used to concentrate 20 wt % NaOH solution entering at 150°F to a 45 wt% NaOH solution exiting at 200°F. What is the pressure in the vapor space of the evaporator
Please answer these simple five questions on fabrication engineering at the micro and nanoscale.
A 30 mol% feed of benzene in toluene is to be distilled in a batch operation. A product having an average composition of 45 mol% benzene is to be produced. Calculate the amount of residue, assuming α = 2.5 and W0 = 100.
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