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The genome of virus SV40 is a circular double-stranded DNA molecule 5 kb long.
a. A 1 kb region is amplified by PCR for four cycles. What fraction of the total DNA does the target sequence constitute? Show your work.
b. After many generations of growth in medium containing 14N, the medium was replaced with new medium containing 15N. After five generations, what is the ratio of light:heavy DNA strands? Show your work.
c. If the number of thymine in the SV40 genome is 1830, what is the percentage of cytosine in the genome? Show your work.
DNA footprinting allows isolation of DNA sequences that bind specific transcription factors. Describe an experimental protocol to identify transcription factors that bind to an isolated DNA sequence.
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When Purified Protein Derivative (PPD) isolated from Mycobacterium tuberculosis was injected into the skin of a patient, it caused an area of hardening 1 cm in diameter. How must this PPD test be interpreted? What caused the result.
A respiratory patient is experiencing trouble breathing and is given supplemental air with the following mixture: 53 percent O2, 2 percent CO2, and 45% N2 at a total pressure of 800 mm Hg.
The research team used the setup to study the incorporation of labelled nucleotides into culture lymphocytes and found that the lymphocytes incorporated the labelled nucleotide at significantly higher level after a pathogen was introduced into the cu..
Review the DNA of the Phenylalanine hydroxylase (PAH) gene in a patient with phenylketonuria revealed a mutation in the protein coding region whose forecasted effect would be to replace the amino acid aspartic acid.
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General characteristics of the four Kingdoms discussed this semester (protists, plants, fungi, animals)
Explain the procedures involved in blood doping. What are the physiological mechanisms behind blood doping that attempt to achieve the desired outcome.
which of the following would you expect to find in normal urine?
Mating between black rats of identical genotype produced offspring as follows: 14 cream colored, 47 black, and nineteen albino.
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