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What is the order of elution of the amino acids

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Reference no: EM131709027

Question - You have a mixture of Glycine, Lysine, and Glutamic acid that you want to separate by cation exchange chromatography. After binding to the column, you apply an increasing pH gradient to elute them (going from pH 0 to 14 for example). What is the order of elution of the amino acids (first, second, last)?

Gly, Glu, Lys

Gly, Lys, Glu

Lys, Gly, Glu

Glu, Gly, Lys

Reference no: EM131709027

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