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Assignment: You suspect that there are mutation in the genes of these sub-strains which are causing the different phenotypes.
Question: For each sub-strain, compare their DNA sequence against the wild-type strain and answer:
1. What's the mutation in the sub-strain's protein CDS? Express your answer in standard notation. Count the adenine in the start codon as 'position 1'. a. Note on standard notation: For example, a Thymine to Adenine mutation at position 200 will be written T200A. You learned this notation for amino acid sequences and the same can be used for nucleotides too.
2. Discuss the effect of this mutation in the protein: a. Did the mutation change the protein primary sequence? If so, how? b. What is the 'name' of this mutation (e.g., silent, missense, nonsense, etc.)? c. Explain how this mutation contributed to the difference (or similarity) between the phenotype of that sub-strain against the wild-type strain?
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