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1. The GFP associated with entry P42212 is derived from which source?
2. Using 7 significant figures, what is the molecular weight of this GFP?
3. Why is the monoisotopic mass different than the average mass?
4. What is the pI of the intact GFP?
5. Removal of the ______ residue(s) would yield a GFP fragment with a lower pI.
6. Which of the 20 standard amino acids is most abundant in GFP?
7. Which of the 20 standard amino acids is least abundant in GFP?
8. GFP contains ____ sulfur atoms, and _____ of these atoms are found in cysteine.
9. According to the primary page for entry P42212 (under the PTM/processing heading), which of the following GFP residues are modified to yield the fluorescent component (or chromophore) that emits green light when excited?
dissolved in 200 mL of solution. What is the pH of the solution? pKa1 = 6.37 and pKa2 = 10.25.
Calculate the detection limit (90% and 95% confidence interval) for a single measurement. Use the equation D.L = (ks)/m
Explain what is the role of the liver in glucose metabolism. Define what is the role of the muscle in glucose metabolism
How many grams of NaCl are contained in 350. mL of a 0.103 M solution of sodium chloride?
What is the new HPO4^2-/H2PO4- ratio and new pH of solution? The pKa of H2PO4- is 6.64. Use henderson-hasselbach. C) Now take a fresh 60mL of the 0.50M pH of 6.83 buffer and add 3.7mL of 1.00M NaOH. Caclulate the new pH of the solution.
is believed to follow the mechanism H2O2 + I- ? H2O + IO- fast H2O2 + IO- ? H2O + O2 + I- slow At 25oC, the first step is fast relative to the second step. What is the rate law predicted by this mechanism?
Consider the reaction that will take place between (S) -2-bromobutane and sodium azide in DMF. A) What is the product of this reaction (give proper stereochemistry of the product formed)
Give the systematic names for the formulas, or the formulas for the names. (The format copper(II) chloride for CuCl2, and using the format CO2 for CO2. Omit the centered dot for hydrates.)
If a reaction was set up at 630K with initial partial pressures of methane of 105.1 torr and of 7.96 torr for I2. Compute the pressures, in torr, of all reactants as well as products at equilibrium.
If 545 mL of oxygen gas is collected at 21ºC and 754 mm Hg, what is its volume at STP?
iron pyrite fes2 is the form in which much of the sulfur exists in coal. in the combustion ofcoal oxygen reacts with
Why when Shutting Down Scanning Electron Microscope do you Turn the magnification to its maximum
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