What is the limit of resolution on this microscope

Reference no: EM13145314

On a particular microscope, the numerical apertures of the condenser and medium power objective lenses are 1.25 and 0.25, respectively. You are supplied with a filter that selects a wavelength of 520nm. What is the limit of resolution on this microscope and will you be able to distinguish two points that are 330nm apart as being separate, or will they blur into one? Later on the same microscope, the high dry lens has a numerical aperture of 0.85. What is the limit of resolution and will you be able to distinguish two points that are 250nm apart as being separate, or will they blur into one?

Reference no: EM13145314

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