What is the limit of resolution

Reference no: EM13161149

a) What is the limit of resolution when using the 40X objective(numerical aperture: 0.65) on the lab's microscopes, in nmand in µm, if you are using light with a wavelength of 550nm? The conversion factor of microscope of 40X is 25μm per oneocular unit.

b)Would you be able to resolve an object that is 0.6 µm indiameter with this 40X objective? Explain your answer.

Reference no: EM13161149

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