What is the length of the wildtype enzyme prior

Assignment Help Biology

Reference no: EM13146075

A few amino acids are post-translationally removed from the C-terminal end of the beta-lactamase enzyme from B. imaginarium (i.e. after translation and release from the ribosome, a protease chews off a few amino acids). The pruified wildtype enzyme is 327 amino acids in length and the sequence of its C-terminal amino acids is shown below aligned with the C-terminal aminoa cids found in a mutant strain of B. imaginarium, which contains of a frameshift mutation that sbolishes processing if its C-terminus. This frameshift mutant has had its translational reading frame altered by insertion or deletion of a single nucleotide.

wild type: S N A K D W

mutant: S N A I R L D W Q M K

a. What was the mutational event that gave rise to the frameshift mutant? Determine precisely where the single nucleotide insertion or deletion occurred in the beta-lacatamase coding sequences.

b. What is the length of the wildtype enzyme prior to processing (how many amino acids)?

c. Deduce, as far as possible, the actual sequence of the C-terminus of the wild-type enzyme prior to processing.

Reference no: EM13146075

Questions Cloud

Calculate the partial pressure (in atmospheres) of each gas : Assuming that the total pressure of the gases is 1.52 atm and that their mole ratio is 94 : 4.0 : 1.5 : 0.50, calculate the partial pressure (in atmospheres) of each gas.

Ratio scale compared to measurements on ordinal scale : What additional information is obtained from measurements on an interval scale compared to measurements on an ordinal scale?

What possible adverse selection and moral hazard problems : Without further restrictions or incentives, what possible adverse selection and moral hazard problems can you envision with this proposal?

What would be the total number of moles of h2so4 needed : Given the reaction: 2Al + 3H2SO4 -> 3H2 + Al2(SO4)3 what would be the total number of moles of H2SO4 needed to react completely with 5.0 moles of Al ?

What is the length of the wildtype enzyme prior : What was the mutational event that gave rise to the frameshift mutant? Determine precisely where the single nucleotide insertion or deletion occurred in the beta-lacatamase coding sequences.

Find the price at which the firm sells the product : Suppose that a firm maximizes its total profits and has a marginal cost (MC) of production of $8 and the price elasticity of demand for the product it sells is (-3). Find the price at which the firm sells the product.

What is the effect of an increase in the price of market : What is the effect of an increase in the price of market goods on a worker's reservation wage, probability of entering the labor force, and hours of work?

Identify independent variable and the dependent variable : Oxytocin were more likely to give their money to a trustee compared to people who inhaled an inactive placebo. For this experimental study, identify the independent variable and the dependent variable.

What is the nucleotide sequence of the wildtype anticodon : At which stop codons would the mutant tRNAglu be able to suppress translational termination? (assume that G-U base pairing is possible at the wobble position)

User Account

All Pages