What is the Kcat for the enzyme

Assignment Help Other Subject

Reference no: EM13255073

Five reaction mixtures containing 0.50 mg of an enzyme are made up with five different substrate concentrations and the initial rates of the reactions were measured. After data analysis, it was determined that the Km was 32 mM and the Vmax was 120 mmol/min.

a) If the molar mass of the enzyme is 140,000 g/mol, what is the Kcat for the enzyme? Is the enzyme catalytically perfect?

b) Suppose the experiment were repeated with five times the amount of enzyme. How would Km and Vmax be affected?

c) A poison is added to the reaction mixture that is known to bind the enzyme at a site different from where teh substrate binds. The poison and the substrate can bind the enzyme simultaneously but the poison prevents the formation of product. Qualitatively, how would the poison affect Km and Vmax?

Reference no: EM13255073

Questions Cloud

The purpose driven life : Pastor Rick Warren, author of The Purpose Driven Life, accepts that homosexuality is a sin, but he also emphasizes his belief in god's love for all people. Why might some members of the religious right reject Warren's formula?

What performance appraisal method is she using : She returns the form to HR, who score it and report the results to her. What performance appraisal method is she using?

Respect to unionization and organized labor : Discuss the historical and future trends toward labor and management conflict resolution with respect to unionization and organized labor.

Calculate the amount of lost energy in jouls : a 8 farad capacitor is charged to 6 volts, at t= 0 this charged capacitor connected in parallel to uncharged capacitor, the value of this uncharged capacitor is 10 farad. calculate the amount of lost energy in Jouls.

What is the Kcat for the enzyme : If the molar mass of the enzyme is 140,000 g/mol, what is the Kcat for the enzyme? Is the enzyme catalytically perfect? Suppose the experiment were repeated with five times the amount of enzyme. How would Km and Vmax be affected?

Financial manager researching investments : Imagine that you are a financial manager researching investments for your client that align with its investment goals. Use the Internet to research any U.S. publicly traded company that you may consider as an investment opportunity for your c..

Atcase is an allosteric enzyme : ATCase is an allosteric enzyme at the beginning of the pathway for the biosynthesis of the nucleotides, CTP and UTP. ATCase is activated in vitro by ATP adn inhibited by CTP.

Solve the vegetarians-and-cannibals puzzle : Solve the vegetarians-and-cannibals puzzle for the case there are three vegetarians and three cannibals to be transported from one side of a river to the other.

Guarantees the purchasing power of the investment : The "Inflation-Plus" CD is the safer investment because it guarantees the purchasing power of the investment.

User Account

All Pages