Reference no: EM132691252
When the only carbon source yeast are provided is an alcohol, obtaining energy from the alcohol requires peroxisomal oxidase enzymes that digest the alcohols. Hydrogen peroxide is a byproduct produced during the alcohol digestion process in the peroxisomes.
A team of Danish scientists grew two strains of the yeast, Hansenula polymorpha, on methanol-based media. Methanol is an alcohol that is processed in the peroxisomes much like ethanol is. One of the strains was a wild-type yeast, while the other one lacked catalase genes. After four days of growth on the methanol-media, the researchers measured the amount of ROS in yeast cells (units = au) from each of the cultures.
1. What is the independent variable?
2. What are the potential values for the independent variable?
3. Is the independent variable categorical or quantitative?
4. What is the dependent variable?
5. What are the potential values for the dependent variable?
6. Is the dependent variable categorical or quantitative?
7. What are the study subjects in this study?
8. What is a reasonable hypothesis for this study?
9. What is logical prediction, given the study design and hypothesis you developed?
10. Is this study experimental or observational?
11. If this study was an experiment, what are the treatments? Label the treatments as either experimental or control.
12. What is the sample size in this study?
13. What are 3 potentially confounding variable that the scientist controlled or should have controlled in the study?
14. Using your knowledge of oxidative stress, how can you explain why accelerated growth rates in an organism might lead to more oxidative stress?