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In mapping a large plasmid you notice that the restriction enzyme HaeII yields three bands, Pst I yields five bands and HaeII+PstI double digestion gives rise to six bands
A) What is the inconsistency of these data and what are two possible explanations?B) How could you distinguish between these possibilities from scrutiny of the gel electrophoresis results?C) You want to ligate the largest fragment generated by PstI with the largest fragment generated by HaeII. However the ends that are generated by the two enzymes are not complementary. What would you need to do in order to carry out the ligation?
Also, how could the P53 protection mechanism negatively influence longevity?
For which case do you expect a stable polymorphism at equilibrium?
Which of the following pairs is most closely related?
Explain why a deficiency either of vitamin B12 or of folate causes red blood cells to be larger than normal.
Question: The genomes of (+) RNA viruses can be translated immediately upon cellular entry, even though they do not possess a 5' 7-methylguanosine cap due to the presence of this structure at the 5' end of the genome [x]
Over the long period of time that life has existed on Earth, there have been number of important or significant innovations including endosymbiosis to create mitochondria and chloroplasts.
At what level of gene expression is the fantasin gene regulated? There are multiple correct answers; please choose one.
Gene expression in prokaryotes involve the following except.
As sound intensity increases, we hear the sound as a louder sound at the same pitch.
An elderly man, Tom, complained to his doctor about intense pain in the joint area of his big toe. The pain would last several days, subside, and then recur weeks later.
The hormone adrenalin increases cardiac output. Briefly explain the parameters that affect cardiac output and discuss how adrenalin can change these parameters.
The seeds of orchids are among the smallest known, with virtually no endosperm and with miniscule seed leaves. As a result, what must one expect to be true of such seeds.
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