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Q1. An enzyme (MW 24 kDa, pI 5.5) is contaminated with two other proteins, one with a similar molecular mass and a pI of 7.0 while the other has a molecular mass of 100 kDa and a pI of 5.4. Suggest a procedure to purify the contaminated enzyme.
Q2. What is the importance of sequestering of glucokinase in nucleus and why is this process activated by fructose-6-phosphate and not by glucose-6-phosphate?
Q3. What are the principles and the purpose of using differential salt precipitation in protein purification?
Hydrogen peroxide, H2O2, is used to disinfect contact lenses. What volume of O2, in mL, of O2(g) at 35°C and 731 mmHg can be liberated from 50.0 mL of an aqueous solution containing 3.00% H2O2 by mass? The density of the aqueous solution of H2O2 i..
The mass to use in the expression for the vibrational frequency of a diatomic molecule is the effective mass μ = mAmB/(mA + mB), where mA and mB are the masses of the individual atoms.
a 0.374g sample of a metallic element m is completely dissolved in 100ml of 0.500n sulfuric acid. the liberated
Gas Chromotography/ Orange oil experiment. Limonene (L) and internal Standard Anisole (A) Were used and needed to find the Wt% of L in a given orange oil sample.
Calculate the final pressure of a gas if a 953-mL sample of the gas at 1.25 atm and 50.0 degrees Celsius is changed to 1.55 L at 75.0 degrees Celsius.Be sure to include units and correct significant figures
Acid-catalyzed dehydration of an alcohol most closely resembles an E1 mechanism when secondary and tertiary alcohols are involved.
H2O is amphoteric meaning it can act as both a Bronsted acid and a Bronsted base.Show that H2O really is amphoteric by writing the relevant equations
Calculate the pH in the titration of 20.0mL of 0.100 M of acetic acid by sodium hydroxide after the addition to the acid solution of
For the reaction a plot of equilibrium constant K vs 1/T Kelvin gave a line with a slop of -2100 K and y intercept of 4.53k. What is the value of standard enthalpy change for the reaction
1-Chloroanthraquinone reacts with methoxide ion to give the substitution product 1-methoxyanthraquinone. Propose a structure for the most stable resonance structure of the intermediate in this reaction.
for the following reactions identify the limiting reactant and the excess reactant. give reasons for your choices.a
Give the cell notation based on the following reaction: Fe(s) + Hg2 ++ (aqueous) = Fe++ (aqueous) + 2Hg (l) the solution concentrations are 0.01 M
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