What is the general rule regarding the correct volume for

Reference no: EM133452133

Questions:

1. What is the general rule regarding the correct volume for cuvettes when using the spectrophotometer?

2. When making 10x TGS in lab, why do you add less than the final volume of u.p. H2O to the solid components?

3. Explain why it is essential to create a serial dilution of your protein sample before performing a protein assay. Do not give me the steps you performed to create a serial dilution. Tell me why you created one.

4. Explain why you used a quartz cuvette for amino acid spectral scans and a plastic cuvette for protein assays.

5. You did 5 scans of your 1% amino acid solution. Make a drawing showing what an ideal scan would look like AND tell me what it can tell you.

6. Protein samples are stored at what temperature?

7. What was the adjusted concentration of the protein standard used to create a dilution series?

Reference no: EM133452133

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