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What is the function of mordant and decolorizer

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1. What is the function of mordant and decolorizer in the gram staining procedure?

2. When looking at the Gram stained sample smear of the mix culture under the microscope, all of the cells appear deep purple. What could have gone wrong?

3. By mistake a lab tech. used safranin as a primary stain and used crystal violet as counter stain. How would your cell appear under the microscope?

4. Why it is important to steam the slide in acid fast and endospore staining procedures

5. Acid fast stains ___________ genus bacteria and detects ___________ in the cell wall of the acid fast positive bacteria.

6. Can you say a positive or negative result after performing the primary stain in the differential staining?

7. Name the few genus of bacteria that can form endospores

8. What makes spores highly resistant to normal staining procedures?

9. Why a piece of paper towel is placed over the slide of endospore and acid fast smear.

10. Why it is important to steam the slide for acid fast and endospore staining?

Reference no: EM132797344

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