What is the function of each enzym

Reference no: EM133471619

Assignment:

For "cleaning up" the PCR products for sequencing you used the ExoSAP-IT® kit (USB Co, Cleveland, OH). This PCR clean up kit contains two enzymes, exonuclease I and shrimp alkaline phosphatase.

What is the function of each enzyme and why do you have to "clean up" the PCR amplified DNA fragments when using the Sanger (di-deoxy-) method for DNA sequencing?

Reference no: EM133471619

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