What is the function of amylase

Reference no: EM131090530

Lab 4: Enzymes

Pre-Lab Questions

1. How could you test to see if an enzyme was completely saturated during an experiment?

2. List three conditions that would alter the activity of an enzyme. Be specific with your explanation.

3. Take a look around your house and identify household products that work by means of an enzyme. Name the products, and indicate how you know they work with an enzyme.

Experiment 1: Enzymes In Food

Data Tables and Post-Lab Assessment

Table 1: Substance vs. Starch Presence

Substance	Resulting Color	Presence of Starch?	Is Amylase present?
Positive Control: Starch
Negative Control:_____ Student must select
Food Product: ______ Student must select
Food Product: ______ Student must select
Saliva

Take a picture of your results. Include a note with your name and date on an index card in the picture. Insert picture here:

Post-Lab Questions

1. What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment?

2. What is the function of amylase? What does amylase do to starch?

3. Which of the foods that you tested contained amylase? Which did not? What experimental evidence supports your claim?

4. Saliva does not contain amylase until babies are two months old. How could this affect an infant's digestive requirements?

5. There is another digestive enzyme (other than salivary amylase) that is secreted by the salivary glands. Research to determine what this enzyme is called. What substrate does it act on? Where in the body does it become activated, and why?

6. Digestive enzymes in the gut include proteases, which digest proteins. Why don't these enzymes digest the stomach and small intestine, which are partially composed of protein?

Experiment 2: Effect of Temperature on Enzyme Activity

Data Tables and Post-Lab Assessment

Table 2: Balloon Circumference vs. Temperature

Tube	Temperature (°C)	Balloon Circumference (Uninflated; cm)	Balloon Circumference (Inflated; cm)
1 - (Cold)
2 - (RT)
3 - (Hot)

Take a picture of your results. Include a note with your name and date on an index card in the picture. Insert picture here:

Post-Lab Questions

1. What reaction is being catalyzed in this experiment?

2. What is the name of the enzyme in this experiment? What is the substrate?

3. What is the independent variable in this experiment? What is the dependent variable?

4. How does the temperature affect enzyme function? Use evidence from your data to support your answer.

5. Draw a graph of balloon diameter vs. temperature. What is the correlation?

6. Is there a negative control in this experiment? If yes, identify the control. If no, suggest how you could revise the experiment to include a negative control.

7. In general, how would an increase in substrate alter enzyme activity? Draw a graph to illustrate this relationship.

8. Design an experiment to determine the optimal temperature for enzyme function, complete with controls. Where would you find the enzymes for this experiment? What substrate would you use?

Reference no: EM131090530

Questions Cloud

Determine the force supported by members bc and df : If the concrete is modeled as a fluid with 90 lb/ft3 specific weight, determine the force supported by members BC and DF.

What is the purpose of cost accounting iss : What is a supply chain? What is the purpose of supply chain management systems? What is the purpose of cost accounting ISs? What is the relationship between CAD and CAM systems?

Why are the federal reserve governors appointed : Why are the Federal Reserve Governors appointed for such long time periods and provide some suggestions of what could happen if they had shorter terms?

Design and implement a simple database application : UMBC - IS 676: Information Integration - Your inventory database contains information about books, and music CDs. The items are stored in different warehouses in the country. Each warehouse stocks different quantities of the products that you sell.

What is the function of amylase : UMUC Biology 102/103 Lab 4: Enzymes. What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment? What is the function of amylase? What does amylase do to starch

Organizations and all stages in the supply chain : Business competition has shifted from company versus company to supply chain versus supply chain. The scope of strategic fit comprises of functions with an organizations and all stages in the supply chain.

Estimate the tip temperature of your pinky finger : Estimate the tip temperature of your pinky finger

What is the critical thickness of insulation : What is the "critical thickness" of insulation, such that if the layer were any thinner, the insulation would actually cause the heat flux to increase?

Find the ratio = convective heat loss /radiation loss : State any assumptions or approximations you make, and find the ratio = convective heat loss / radiation loss?

User Account

All Pages