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MCB100A_F2015_PS9.pages
8. While working at a biotech startup, you isolate an inhibitor against an important drug target, the dimeric protein known as Enzyme X. Answer the following:
a. The binding of your inhibitor to Enzyme X appears to involve an allosteric mechanism and two different KDs are measured: KD1 = 10 nM and KD2 = 1 nM. What is the fraction of protein with ligand bound when [inhibitor] = 100 nM?
b. Sketch the binding isotherm for each single binding site as well as the cooperative binding isotherm for the inhibitor below. Label the axes and note on the plot where one can determine the Hill Coefficient and whether this specific binding mechanism displays positive or negative cooperativity.
c. The inhibitor is extremely effective in initial drug trials, but in some patients mutations mutation in Enzyme X arise that reduces its potency. These mutations are found on the dimer interface and reduce the Hill Coefficient to nH = 0.25. What is the new KD2 and what is the fraction of protein with bound drug at 100 nM now?
A 1.20 M solution of citric acid (H3C6H5O7) in water has a density of 1.10 g/cm3. Calculate the normality. Citric acid has three acidic protons.
1. caculate the volume of the acid soultion needed to completly neutralize the base in the flask 2. Caculate the pH for (a) the inital base solution in the flask; (b) the point at which 20mL of the acid has been added; (c) the equivalence point, a..
What is the pH of the buffer that result when 12 g of NaH2Po4 and 8.00 g of NaHPO4 are diluted with water to a volume of 0.50 L ? ( Ka of H2PO4- = 6.2*10^-8 , the molar masses of NaH2PO4 and Na2HPO4 are 120.0 g/mol and 142.0 g/mol respectively )
Determine whether each of the given amounts of solid will completely dissolve in the given amount of water at the indicated temperature.
a solution of ethanol c2h5oh in water is prepared by dissolving 62.0 ml of ethanol density 0.79 gcm3 in enough water
Assuming the activation energies are equal, which of the following reactions will proceed at a higher rate at 50 degrees celsius? WHY?
A compound gave an orange precipitate when reacted with 2,4 -DNP and showed a strong absorbance. The Tollens test shows a negative result
Calculate the number of moles needed to provide 20g of glucose from a 60% glucose solution
a solution contains 0.115 mol h2o and an unknown number of moles of sodium chloride. the vapor pressure of the solution
Give the overall reaction for the dehydration of cyclohexanol.2. IR spectroscopy will be used to verify the isolated compound is the correct compound (cyclohexene). What stretches (presence and absence) will distinguish the product
Which one of these equations describes a redox reaction? 2Al+3H2SO4(aq)-->Al2(SO4)3(aq)+3H2(g), 2KBr(aq)+Pb(NO3)2(aq)-->2KNO3(aq)+PbBr2(s), CaBr2(aq)+H2SO4(aq)-->CaSO4(s)+2HBr(g), H+(aq)+OH-(aq)-->H2O(1), CO.-(aq)+HSO4-(aq)-->HCO3-(aq)+SO42-(aq)
If you were cooking food in boiling water on the stove, could you increase the speed of cooking by increasing the heat. Why or why not. What is effect does adding salt to water have on the boiling point. Explain
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